The most amazing antioxidant and detoxifier: sulforaphane in broccoli seed extract

According to the National Academy of Science, broccoli and broccoli sprouts contain the largest amounts of glucosinolates and sulforaphane, which are potent inducers of enzymes that protect against chemical carcinogens and involve in liver detoxification.

In its raw form, broccoli has a substance called myrosinase inside it, which converts to sulforaphane when eaten. But if it’s cooked for more than a four-minute steam, broccoli rapidly loses this compound, along with it many of the health benefits.

You can pair the broccoli with broccoli sprouts, mustard, horseradish, or wasabi—the spicier the better. Such combinations will provide more sulforaphane than any one on their own. If you’re not a fan of culinary spice, you can use radishes, cabbage, arugula, watercress, and Brussels sprouts.

Sulforaphane from broccoli is efficiently and rapidly absorbed in our small intestine, then distributed throughout our body, providing really potent health benefits to many vulnerable tissues.

The detox mechanism—Phase I and Phase II

The mechanism in which our cells detoxify potentially harmful compounds—including many cancer-causing substances (carcinogens)—involves a so-called Phase I component in the liver that’s associated with the Cytochrome P450 system. It is then followed by a Phase II component where the intermediate compound produced by Phase I is bound in a way that permits ready excretion.

For a safe and efficient detoxification, toxins should ideally undergo a relatively slow Phase I reaction followed by a more rapid Phase II in order to prevent accumulation of the Phase I metabolite, which can be more toxic than its precursor.

Sulforaphane from broccoli extracts stimulates this system perfectly and powerfully. Sulforaphane can slightly slow down some parts of Phase I, and also acts as an inducer of Phase II.

The indole compounds (such as I-C-3 and DIM) from mature broccoli stimulate both Phase I and Phase II, which is less than ideal but still good for us.

Of clinical significance is the finding that Phase II enzymes have a relatively long half-life, so that up-regulated expression of these enzymes can remain for several days. Thus just three serves of broccoli or sulforaphane per week can have very protective effects, as shown in hundreds of population studies.

Phase II enzymes are induced by a component called Nrf2, and sulforaphane is a potent Nrf2 inducer. Consider Nrf2 as responsible for induction of a myriad of cellular defences [2b]. The downstream enzyme products of Nrf2 target genes are efficient and versatile. They include the glutathione and thioredoxin systems, the major cellular reducing or anti-oxidant systems in our body.

Several reasons explain why Nrf2 induced enzymes are efficiency and versatility: firstly they are not consumed as they work as are direct-acting antioxidants such as vitamin C and E; secondly their duration of action is long with half-lives measured in days; and thirdly they restore the internally produced direct-acting antioxidants like coenzyme Q10 and the vitamin E by returning them to the active, or so-called reduced state.

Another interesting interaction is with Vitamin D. Vitamin D’s protective effects on human cells are well recognized, and like sulforaphane it touches beneficially upon many hundreds of genes. It is nutritionally significant that the vitamin D receptor (VDR) is a Nrf2 target gene inducible by sulforaphane; in turn, vitamin D can increase Nrf2 expression so these two compounds are synergistically beneficial to health.

So we have established some of the beneficial anti-aging effects via Nrf2 stimulation: animal and human studies show significant decline in Nrf2 activity between youth and old age.

Crucifer sprouts may protect against Carcinogenic Development

A related enzyme stimulated by Nrf2 is NQO1. The activity of this is measurable and is used as a rapid screening procedure and a biomarker of the anti-carcinogenic activity of phytochemicals. [2c]

When several crucifers were compared for their Nrf2/NQO1 inducer effect, sulforaphane returned ~33,000 units of NQO1 inducer activity per gram of fresh weight for broccoli, cabbage returned ~11,000 units, kale returned ~10,000 units, while turnip returning just ~2,000 units. [2c].

“Large quantities of inducers of enzymes that protect against carcinogens can be delivered in the diet by small quantities of young broccoli sprouts which contain as much inducer activity as 10–100 times larger quantities of mature vegetables” [2]. Small quantities of crucifer [broccoli] sprouts may protect against the risk of cancer as effectively as much larger quantities of mature vegetables of the same variety. [2]

In short, Broccoli is the best mature vegetable to eat, but the sprouts are even better!

As we will see from research below, our eyes, brain, lungs, breasts, colon and prostate as well as liver and kidneys gain benefits from the protection that sulforaphane produce in our cells.

The chemoprevention properties of sulforaphane against cancer are through both “blocking” and “suppressing” effects [3]. The “blocking” function of sulforaphane is achieved through inhibiting Phase I metabolism enzymes that convert pro-carcinogens to carcinogens and inducing Phase II metabolism enzymes that promote excretion of carcinogens [3, 33].

Subsequent studies revealed the “suppressing” effects of sulforaphane in modulating diverse cellular activities to inhibit the growth of transformed cells [3, 4, 5, 7, 8, 9, 32].

So sulforaphanes block carcinogens [33] and suppress cells becoming cancerous, as we shall see in following research. [5b, 32].

The health benefits:

1. Reduced breast cancer cells

The existence of cancer stem cells (CSCs) in breast cancer has profound implications for cancer prevention. In one study, they evaluated sulforaphane for its efficacy to inhibit breast cancer stem cells and its potential mechanism. Breast cancer is initiated from and maintained by a small population of breast cancer stem cells. Their studies were both in the test tube and in live mice. The test tube assay and another test called mammosphere formation assay showed that sulforaphane inhibited breast cancer stem cells in vitro (i.e. “in glass” a lab situation), and eliminated breast cancer stem cells in vivo (means in the living animal). [5]

Study conclusions: “These studies support the use of sulforaphane for breast cancer chemoprevention”. [5]

In a subsequent pilot study, eight healthy women undergoing reduction mammoplasty were given a single dose of a broccoli sprout preparation containing 200 mmol of sulforaphane. Following oral dosing, sulforaphane metabolites were readily measurable in human breast tissue enriched for epithelial cells. These findings provide a strong rationale for evaluating the protective effects of a broccoli sprout preparation in clinical trials of women at risk for breast cancer.” [5b]

There are many other positive breast-cancer related studies so these are a sample only.

2. Reduced prostate cancer cells in animal studies

Prostate and colon cancer have both also been shown to be responsive to sulforaphanes. Diets high in cruciferous vegetables are associated with lower risk of incidence of prostate cancer, including aggressive forms of this disease. One study showed that men with the glutathione S transferase M1 (GSTM1)-present genotype and high broccoli intake had the greatest reduction in risk— basically reducing risk of prostate cancer by half in this vulnerable group. [7]

The findings provide evidence that two or more servings per month of cruciferous vegetables may reduce risk of prostate cancer, especially among men with GSTM1-present alleles, and are consistent with a role of dietary sulforaphane as chemopreventive agents against prostate cancer.[7]

Human intervention studies with sulforaphane show that it induced cell death in human prostate cancer cells. This was initiated by reactive oxygen species—meaning sulforaphane kills cancer cells in rats by oxidizing them to death.[8] It was also found that taking N-acetyl cysteine at the same time counteracted this beneficial effect. [8]

3. Protects our lungs from pollution

Broccoli extract also protects the lungs, potentially from cancer, certainly from pollutants.

As we now know naturally occurring sulforaphane from broccoli sprouts induce a potent increase in antioxidant Phase II enzymes such as glutathione, but that occurs not only in the liver but also in lung airway cells. A study conclusion stated: “Oral sulforaphane safely and effectively induces mucosal Phase II enzyme expression in the upper airway of human subjects. This study demonstrates the potential of antioxidant Phase II enzymes induction in the human airway as a strategy to reduce the inflammatory effects of oxidative stress, a novel therapeutic strategy for oxidant induced airway disease.“ Similar effects are gained with wasabi, or horseradish, both cabbage family plants with potent sulforaphane nasal clearing and detoxing benefits. [9]

Translated, this means sulforaphane protects the lungs from pollution and smoke.

4. Protects our eyes From ageing

A study suggests that sulforaphane enhances the ability of human retinal pigment epithelial cell against oxidative stress. The results show that sulforaphane may be a valuable supplement for preventing and retarding the development of Age-Related Macular Degeneration. [10]

5. Protects our kidneys and bladder

These protective effects are mediated by activating Nrf2, a protein that regulates the expression of antioxidant proteins that protect against oxidative damage triggered by injury and inflammation, prevent oxidative and inflammatory stressors to the kidneys and bladder, two organs sensitive to pollution induced damage and cancers. Diabetic Neuropathies and kidney damage from poisons may also be managed with sulforaphanes. [11-18]

So it turns out that Broccoli sprouts are the richest food source of sulforaphane, and these are proven to be cancer protective, and as we will see protect our eyes, kidneys, bladder brain, heart and nerves from oxidative damage.

Furthermore sulforaphanes protects the brain.

6. Protects our brain

Broccoli sprouts have helped many autistic young men, through improvements in detoxification, which is well documented as dysfunctional in autistics. Broccoli sprouts seem to help according to one study.

In a placebo-controlled, randomised, double-blind clinical trial, daily oral administration of broccoli sprout extract for 18 weeks to 29 young men with autism substantially improved behaviour compared with 15 placebo recipients.

Behaviour was quantified by both parents/caregivers and physicians by three widely accepted measures. Regarding social behaviour 46% (12 of 26), 54% (14 of 26), and 42% (11 of 26) of sulforaphane recipients were much or very-much improved on social interaction, aberrant behaviour, and verbal communication, respectively, compared with 0%, 9%, and 0% respectively, for placebo recipients. There were similar percentages of changes in aberrant behaviours in the young autistic men.[19] Extensive evidence shows that sulforaphane counteracts many of the same biochemical and molecular abnormalities associated with ASD, including oxidative stress and reduced antioxidant capacity, defects in glutathione synthesis, and neuro-inflammation. [20]

We can expect that broccoli sprout extract is able to protect nerves and improve the brains function for us all from these and other studies. For a technical look into the brain protective effects see the following article [21] which in summary says: “ the observed protective effects of sulforaphane against brain oxidative stress are mainly associated with Nrf2 activation and the resulting upregulation of antioxidant cytoprotective proteins and elevation of glutathione.

The benefits of sulforaphane have even been shown to repair a damaged blood brain barrier (so called “leaky brain”). [22-23]

7. Heals Traumatic Brain Injury (TBI)

Traumatic Brain Injury – concussion – increases long-term mortality and reduces life expectancy. It is associated with increased incidences of seizures, sleep disorders, neurodegenerative diseases, neuroendocrine dysregulation, and psychiatric diseases, as well as non-neurological disorders such as sexual dysfunction, bladder and bowel incontinence, Alzheimer’s disease and systemic metabolic dysregulation that may arise and/or persist for months to years’ post-injury.

Sulforaphane enhances decreases cerebral edema (swelling) following traumatic brain injury. [27] Administration of sulforaphane to traumatic brain injured mice resulted in an up-regulation of Nrf2-dependent antioxidant enzymes and a reduction of oxidative damage after Traumatic Brain Injury (TBI). In accordance with these biochemical changes, sulforaphane also significantly reduced neuronal death, contusion volume, and neurological dysfunction after TBI. [28]

Sulforaphanes protect against the damaging effects of amyloid beta, the key compound related to Alzheimer’s disease. There is some hope from animal studies of a protective benefit in Alzheimer’s in that they showed amelioration of cognitive function in behaviour tests. Animal studies also showed the antioxidant glutathione had beneficial effects in Parkinson’s Disease models. [29-32]

8. Benefits the cardiovascular system

Suffice it to say that of all vegetables studied it is the cabbage/broccoli family with the most protective effects, according to a 2015 study “Phyto-chemical compounds and protection from cardiovascular diseases: a state of the art”. [32b]

Is sulforaphane more powerful than antioxidant vitamins?

Yes, by far.

What has become clear is that attempts to counter oxidative stress by “antioxidant” vitamin supplementation don’t prove well in studies. [34] In a meta-analysis by the Cochraine group they found that synthetic antioxidants found in many supplements fail to produce disease preventing results when studied: “The slightly increased risk of mortality [3%] was associated with beta-carotene and possibly [synthetic] vitamin E and [synthetic] vitamin A. The current evidence does not support the use of [synthetic man made] antioxidant supplements in the general population or in patients with various diseases”. [34]

That is a strong science-based statement by this respected Cochraine group. See the reference if you would like to see how extensive the research is behind the statement.

Yet we know that plant-based antioxidants do protect from illness, and sulforaphanes and the turmeric extract curcumin are the most powerful, as found in many studies some here reported.

Natural carotenoids and natural vitamin E and C when found in food come in synergistic forms of several compounds – and are much more complex than synthetic vitamin A, synthetic beta carotene, and man-made tocopherols (most vitamin E) found in a standard vitamin supplement.

I think the routine vitamin supplement if it is an antioxidant formula and not derived from foods is a waste of money.

On the other hand, sulforaphane from broccoli and curcumin from turmeric are among the best documented protective health inducers with proven anticancer and other health benefitting properties, and whose molecular mechanisms have been extensively investigated [32-38].

The main purpose of one mice study was to compare vitamin supplements with food-based antioxidants, showed the only sulforaphane and phytonutrient-based approach worked. [39]

For a full review article see sulforaphane and Other Nutrigenomic Nrf2 Activators: Can the Clinician’s Expectation Be Matched by the Reality? [32]

The comments by these experts state: “Broccoli-derived sulforaphane emerges as a phytochemical with the capability of … favourably modifying genes associated with chemoprevention”. Compared with widely used phytochemical-based supplements like curcumin, silymarin, and resveratrol, sulforaphane more potently activates Nrf2 to induce the expression of a battery of cytoprotective genes. Both its high bioavailability and significant Nrf2 inducer capacity contribute to the therapeutic potential of sulforaphane-yielding supplements.” [32]


Compared with widely used vitamins or other phytonutrient or herbal supplements like curcumin, silymarin (milk thistle), and resveratrol (red wine extract), sulforaphane more potently activates Nrf2 to induce the expression of a battery of protective genes and improves glutathione levels as part of this effect. Glutathione is the master anti-oxidant gobbling up and eliminating many pollutants, including air pollutants and heavy metals.

Broccoli seed extracts are the most potent source of sulforaphanes, and are easily obtained in capsules. Two daily, or even most days a week are highly effective at raising our cellular defences, so vital in polluted environments.

Curcumin, the turmeric extract, is also excellent as a phytonutrient. Curcumin assists sulforaphane, and it has more anti-inflammatory benefits, also thinning the blood in a safer way than does aspirin.

Vitamin D3 is also synergistic with sulforaphane, with its own immune protective and anti-cancer benefits being well established by hundreds of studies.

I believe there is no other supplement can protect our health better than sulforaphane from broccoli seed extract.

1. T. A. Shapiro, J. W. Fahey, K. L. Wade, K. K. Stephenson, and P. Talalay, “Human metabolism and excretion of cancer chemoprotective glucosinolates and isothiocyanates of cruciferous vegetables,”Cancer Epidemiology Biomarkers and Prevention, vol. 7, no. 12, pp. 1091–1100, 1998.
2. Jed W. Fahey, Yuesheng Zhang, and Paul Talalay. Broccoli sprouts: An exceptionally rich source of inducers of enzymes that protect against chemical carcinogens. Proceedings of the National Acadamy of Science September 16, 1997 vol. 94no. 19 10367-10372.
[2b] R. Hu, C. Xu, G. Shen et al., “Gene expression profiles induced by cancer chemopreventive isothiocyanate sulphoraphane in the liver of C57BL/6J mice and C57BL/6J/Nrf2 (-/-) mice,” Cancer Letters, vol. 243, no. 2, pp. 170–192, 2006.
[2c] K. N. Lewis, J. Mele, J. D. Hayes, and R. Buffenstein, “Nrf2, a guardian of healthspan and gatekeeper of species longevity,” Integrative and Comparative Biology, vol. 50, no. 5, pp. 829–843, 2010.
3. Clarke JD, Dashwood RH, Ho E. Multi-targeted prevention of cancer by sulphoraphane. Cancer Lett.2008;269:291–304. [PMC free article] [PubMed]
4. Zhang Y, Tang L. Discovery and development of sulphoraphane as a cancer chemopreventive phytochemical. Acta Pharmacol Sin. 2007;28:1343–54. [PubMed]
5. Clin Cancer Res. 2010 May 1; 16(9): 2580–2590.. doi: 10.1158/1078-0432.CCR-09-2937
5b. B. S. Cornblatt, L. Ye, A. T. Dinkova-Kostova et al., “Preclinical and clinical evaluation of sulphoraphane for chemoprevention in the breast,” Carcinogenesis, vol. 28, no. 7, pp. 1485–1490, 2007.
6. Gasper AV, Al-Janobi A, Smith JA, Bacon JR, Fortun P, Atherton C, Taylor MA, Hawkey CJ, Barrett DA, Mithen RF. Glutathione S-transferase M1 polymorphism and metabolism of sulphoraphane from standard and high-glucosinolate broccoli. The American journal of clinical nutrition. 2005;82(6):1283–1291. 82/6/1283 [pii] [PubMed]
7. Joseph MA, Moysich KB, Freudenheim JL, Shields PG, Bowman ED, Zhang Y, Marshall JR, Ambrosone CB. Cruciferous vegetables, genetic polymorphisms in glutathione S-transferases M1 and T1, and prostate cancer risk. Nutr Cancer. 2004;50(2):206-13. PMID:15623468.
8. Shivendra V. Singh, Sanjay K. Srivastava, Sunga Choi, Karen L. Lew, Jedrzej Antosiewicz, Dong Xiao, Yan Zeng, Simon C. Watkins, Candace S. Johnson, Donald L. Trump, Yong J. Lee, Hui Xiao and, Anna Herman-Antosiewicz. sulphoraphane-induced Cell Death in Human Prostate Cancer Cells Is Initiated by Reactive Oxygen Species. Journal of Biological Chemistry 2005: 280, 19911-19924.
9. Riedl MA, Saxon A, Diaz-Sanchez D. Oral sulphoraphane increases Phase II antioxidant enzymes in the human upper airway. Clin Immunol. 2009;130 (3):244–251. doi: 10.1016/j.clim.2008.10.007.[PMC free article] [PubMed] [Cross Ref]
10. Oxidative Medicine and Cellular Longevity Volume 2013 (2013), Article ID 413024, 13 pages
11. Cui W, et al Prevention of diabetic nephropathy by sulphoraphane: possible role of nrf2 upregulation and activation . Oxid Med Cell Longev. (2012)
12. Alp H, et al Effects of sulphoraphane and curcumin on oxidative stress created by acute malathion toxicity in rats . Eur Rev Med Pharmacol Sci. (2012)
13. Chung SD, et al Activating nrf-2 signaling depresses unilateral ureteral obstruction-evoked mitochondrial stress-related autophagy, apoptosis and pyroptosis in kidney . PLoS One. (2012)
14. Zheng H, et al Therapeutic potential of Nrf2 activators in streptozotocin-induced diabetic nephropathy . Diabetes. (2011)
15. Yoon HY, et al sulphoraphane protects kidneys against ischemia-reperfusion injury through induction of the Nrf2-dependent phase 2 enzyme . Biochem Pharmacol. (2008)
16. Guerrero-Beltrán CE, et al sulphoraphane protects against cisplatin-induced nephrotoxicity . Toxicol Lett. (2010)
17. Cheung KL, Khor TO, Kong AN Synergistic effect of combination of phenethyl isothiocyanate and sulphoraphane or curcumin and sulphoraphane in the inhibition of inflammation . Pharm Res. (2009)
18. Shapiro TA, et al Chemoprotective glucosinolates and isothiocyanates of broccoli sprouts: metabolism and excretion in humans . Cancer Epidemiol Biomarkers Prev. (2001)
19. Proc Natl Acad Sci U S A. 2014 Oct 28; 111(43): 15550–15555. doi: 10.1073/pnas.1416940111.
20. – r12 – r13 – r14 – r15–16
21. Andrea Tarozzi, Cristina Angeloni, Marco Malaguti, Fabiana Morroni, Silvana Hrelia, and Patrizia Hrelia, “sulphoraphane as a Potential Protective Phytochemical against Neurodegenerative Diseases,” Oxidative Medicine and Cellular Longevity, vol. 2013, Article ID 415078, 10 pages, 2013. doi:10.1155/2013/415078
22. J. Zhao, A. N. Moore, J. B. Redell, and P. K. Dash, “Enhancing expression of Nrf2-driven genes protects the blood-brain barrier after brain injury,” Journal of Neuroscience, vol. 27, no. 38, pp. 10240–10248, 2007.
23. J. Xiang, G. N. Alesi, N. Zhou, and R. F. Keep, “Protective effects of isothiocyanates on blood-CSF barrier disruption induced by oxidative stress,” American Journal of Physiology, vol. 303, no. 1, pp. R1–R7, 2012.
24. Z. Ping, W. Liu, Z. Kang et al., “sulphoraphane protects brains against hypoxic-ischemic injury through induction of Nrf2-dependent phase 2 enzyme,” Brain Research, vol. 1343, pp. 178–185, 2010.
25. L. Soane, W. Li Dai, G. Fiskum, and L. L. Bambrick, “sulphoraphane protects immature hippocampal neurons against death caused by exposure to hemin or to oxygen and glucose deprivation,” Journal of Neuroscience Research, vol. 88, no. 6, pp. 1355–1363, 2010.
26. C. A. Danilov, K. Chandrasekaran, J. Racz, L. Soane, C. Zielke, and G. Fiskum, “sulphoraphane protects astrocytes against oxidative stress and delayed death caused by oxygen and glucose deprivation,” Glia, vol. 57, no. 6, pp. 645–656, 2009
27 J. Zhao, A. N. Moore, G. L. Clifton, and P. K. Dash, “sulphoraphane enhances aquaporin-4 expression and decreases cerebral edema following traumatic brain injury,” Journal of Neuroscience Research, vol. 82, no. 4, pp. 499–506, 2005.
28. Yuan Hong, Wei Yan, Sheng Chen, Chong-ran Sun and Jian-min Zhang. The role of Nrf2 signaling in the regulation of antioxidants and detoxifying enzymes after traumatic brain injury in rats and mice. Acta Pharmacologica Sinica (2010) 31: 1421–1430; doi: 10.1038/aps 2010.101; published online 18 Oct 2010
29. H. M. Park, J. A. Kim, and M. K. Kwak, “Protection against amyloid beta cytotoxicity by sulphoraphane: role of the proteasome,” Archives of Pharmacal Research, vol. 32, no. 1, pp. 109–115, 2009.
30. H. V. Kim, H. Y. Kim, H. Y. Ehrlich, S. Y. Choi, D. J. Kim, and Y. Kim, “Amelioration of Alzheimer’s disease by neuroprotective effect of sulphoraphane in animal model,” Amyloid, vol. 20, no. 1, pp. 7–12, 2013.
31. F. Morroni, A. Tarozzi, G. Sita, et al., “Neuroprotective effect of sulphoraphane in 6-hydroxydopamine-lesioned mouse model of Parkinson’s disease,” Neurotoxicology, vol. 36, pp. 63–71, 2013.
32. Christine A. Houghton, Robert G. Fassett, and Jeff S. Coombes sulphoraphane and Other Nutrigenomic Nrf2 Activators: Can the Clinician’s Expectation Be Matched by the Reality? Oxidative Medicine and Cellular Longevity Volume 2016 (2016), Article ID 7857186, 17 pages
[32b] B. Pagliaro, C. Santolamazza, F. Simonelli, and S. Rubattu, “Phytochemical compounds and protection from cardiovascular diseases: a state of the art,” BioMed Research International, vol. 2015, Article ID 918069, 17 pages, 2015
33. Y.-J. Surh, J. K. Kundu, and H.-K. Na, “Nrf2 as a master redox switch in turning on the cellular signaling involved in the induction of cytoprotective genes by some chemopreventive phytochemicals,” Planta Medica, vol. 74, no. 13, pp. 1526–1539, 2008.
34. Bjelakovic G, Nikolova D, Gluud LL, Simonetti RG, Gluud C. Antioxidant supplements for prevention of mortality in healthy participants and patients with various diseases. Cochraine Database of Systematic Reviews, 14 March 2012.
35. Talalay P, Fahey JW. Phytochemicals from cruciferous plants protect against cancer by modulating carcinogen metabolism. J Nutr. 2001;131(Suppl):3027S–3033S. [PubMed]
36. Dinkova-Kostova AT, Massiah MA, Bozak RE, Hicks RJ, Talalay P. Potency of Michael reaction acceptors as inducers of enzymes that protect against carcinogenesis depends on their reactivity with sulphhydryl groups. Proc Natl Acad Sci USA. 2001;98:3404–3409. [PMC free article] [PubMed]
37. Biswas SK, McClure D, Jimenez LA, Megson IL, Rahman I. Curcumin induces glutathione biosynthesis and inhibits NF-kappaB activation and interleukin-8 release in alveolar epithelial cells: mechanism of free radical scavenging activity. Antioxid Redox Signal. 2005;7:32-41. [PubMed]
38. Dinkova-Kostova AT, Holtzclaw WD, Cole RN, Itoh K, Wakabayashi N, Katoh Y, Yamamoto M, Talalay P. Direct evidence that sulphydryl groups of Keap1 are the sensors regulating induction of phase 2 enzymes that protect against carcinogens and oxidants. Proc Natl Acad Sci USA. 2002;99:11908–11913.
39. Rebrin I, Zicker S, Wedekind KJ, Paetau-Robinson I, Packer L, Sohal RS. Effect of antioxidant-enriched diets on glutathione redox status in tissue homogenates and mitochondria of the senescence-accelerated mouse. Free Radic Biol Med. 2005 Aug 15; 39(4): 549–557.

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